Cloning and Expression Analysis of Tomato <i>SlSPS1</i> Gene

Sucrose is the main product of plant photosynthesis and participates in many physiological biochemical reactions plants. phosphate synthase one key rate-limiting enzymes necessary for sucrose to enter various metabolic pathways, its activity directly reflects ability synthesis In order study mechanism tomato, this cloned a gene from tomato pulp named it&nbsp; SlSPS1 . Through bioinformatics analysis, it was found that cDNA 3488bp length, encoding 1054 amino acids, there are 3 functional domain. There 4&nbsp; SPS &nbsp;genes their homology relatively high. Salicaceae crops, protein closely related potatoes, peppers eggplants. The results tissue-specific analysis that&nbsp; &nbsp;was expressed all tissues, among which expression high seedling stage different development stages fruit. further growth accumulation, an overexpression knockout vector of&nbsp; constructed transformed into Micro-Tom obtain transgenic positive lines. Analysis level &nbsp;on obtained lines showed levels at stage, fruit green ripe ripening were significantly up-regulated compared with wild type, enzyme content increased. Compared &nbsp;SlSPS1 &nbsp;at line down-regulated, reduced. As &nbsp;in verification needed future.